Breve descripción acerca de los cromosomas politénicos de Drosophila melanogaster. View Notes – Laboratorio cromosomas politénicos (1).docx from BIO at Javeriana University. Laboratorio Identificacin de Cromosomas Politnicos de. Una herramienta importante para estos estudios es el etiquetado de los cromosomas politénicos con anticuerpos frente a la enzima, el factor de transcripción.

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Repita los pasos 4. Agradecemos a la Sra.

Preparación de los cromosomas politénicos de Drosophila Calabazas para el Etiquetado de anticuerpos

You must be signed in to post a comment. You will only be able to see the first 20 seconds. When you put them on the coverslip and then pick up the coverslip with the slide the chromosomes may float away to the edges crpmosomas the cover slip if there is too much liquid present. Skip to content Biology. Get cutting-edge science videos from J o VE sent straight to your inbox every month.

The dissection forceps we use were mis-identified: Please check your Internet connection and reload this page. Fill out the form below to receive a free trial or learn more about access: If politehicos doesn’t help, please let us know.


Cromosomas politénicos |

Warm to disperse the Triton X This may be a dumb question but when you put the tissue in Fixative 1 the Triton will lyse the membrane scorrect?

Try placing them on the coverslip in a smaller drop of liquid and being careful about how you apply the pressure when you squash. Coloque un segundo Kim-borrar en la parte superior y aplanar los cromosomas, colocando el pulgar sobre el lugar donde se coloca el cubreobjetos y presionar con firmeza, evitando cualquier movimiento horizontal del cubreobjetos que se cortelos cromosomas.

Please sign in or create an account. A subscription to J o VE is required to view this article. You are right about the Triton.

Deficiencia intelectual – catarata – cifosis

Unable to load video. Is it the PFA?

Las modificaciones de este procedimiento se describen en detalle en Johansen et al. Click here for the english version.

If you have any question you can send me another e-mail. We recommend downloading the newest version of Flash here, but we support all versions 10 and above. A squash that is too rapid or hard may also cause the chromosomes to float away. The trick is to leave them in just long enough.

Examinar el portaobjetos una vez que el hielo se ha sublimado lejos de confirmar que el tejido adherido a la diapositiva y no quedarse con el cubreobjetos.


Please recommend JoVE to your librarian. That is the volume I prepare each time, usually enough for two or three prep. An unexpected error occurred. Lugar pesos g en el cubreobjetos de las diapositivas.

Infográfico: Cromosomas politénicos ~ Ciber-Genética

If you leave the glands in too long they will fall apart. Fill out the form below to receive a free trial or learn more about access:. Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes.

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Do you have any advice? My ceomosomas is that they may be getting flushed out during my transfers. If you are seeing few salivary gland chromosome sets you may be losing them when you squash. For other languages click here. Caliente para dispersar a la Triton X y su uso dentro de una hora. If the problem continues, please let us know and we’ll try to help.